Fig. 4
From: Enterococcus faecalis promotes the progression of colorectal cancer via its metabolite: biliverdin
BV promotes proliferation and angiogenesis in vivo. A Representative images of subcutaneous tumors in nude mice injected HCT116 cells treated with or without BV. Both the volume and weight of subcutaneous tumor were shown in the right panel. B PI3K/AKT/mTOR pathway was activated in mice tumor by western blot analysis. C The concentration of IL-8 and VEGFA in mice tumors were detected by ELISA. D The growth of human colon cancer organoids was assessed after 10 days of BV treatment or not. E IHC analysis demonstrated the expression of CD31 in subcutaneous tumors of nude mice. Bars of the right panel represent the microvascular density. Scale bar represents 100 μm. F, G The expression of Ki67 and CD31 in subcutaneous tumors of nude mice and organoid models were assessed by immunofluorescence staining. Representative images were shown. Scale bar represents 50 μm and 20 μm, respectively. *, P < 0.05; **, P < 0.01; ***, P < 0.001