Fig. 8

Confirmation of hub MitoDEGs expression and association with cardiac function in DCM rats. a–j General biological and echocardiography features of DCM rats; k Hub MitoDEGs mRNA expression of CON and DCM rats; l, m Protein levels of Pdk4, Hmgcs2, Decr1 and Ivd by western blotting, and quantitative analysis in cardiac tissues; n Immunostaining of Pdk4, Hmgcs2, Decr1 and Ivd protein expression. o Correlations between Pdk4, Hmgcs2, Decr1, Ivd mRNA levels and cardiac functional parameters in CON and DCM rats, including: EF%, FS%, LVIDs(mm). Mean ± SD, n = 4 rats per group. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 vs. control group. ns no significance