Fig. 5
From: Sirt3 restricts tumor initiation via promoting LONP1 deacetylation and K63 ubiquitination
A–C STAM, Hrs and hHR23 were knocked down respectively in HEK293T cells. Then, the protein level of LONP1 was detected. D, E HA-Ub, HA-K63-Ub, HA-Ub-K63R plasmid were transfected in LONP1 WT cells and LONP1 K145Q mutation cells respectively. Cells were immunoprecipitated with LONP1 antibody followed by Western blotting. F HA-Ub plasmid was transfected in Flag-tagged LONP1 WT, K145Q and K145R mutation cells respectively. Ub level was detected by WB