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Fig. 3 | Journal of Translational Medicine

Fig. 3

From: Sirt3 restricts tumor initiation via promoting LONP1 deacetylation and K63 ubiquitination

Fig. 3

Sirt3 modulates the deacetylation and expression of LONP1. A The whole cells were lysed after treatment of 10 mM NAM for 0, 2, 4, 6 and 8 h respectively. WB detection of LONP1 and Sirt3 showing NAM treatment increased the expression of LONP1. B Sirt3 decreased the protein level of LONP1. The treatment of NAD+ (agonist of Sirt3) decreased the level of LONP1, while its was resisted by NAM. C WB detection of LONP1 after treatment of cycloheximide (75 μg ml−1) with of without 10 mM NAM treatment. D, E The cytoplasmic protein levels of LONP1 were increased after NAM treatment or Sirt3-knockdown. The cytoplasm and mitochondria were isolated and the protein levels of LONP1 was detected by WB in cells treated with NAM D and cells stably knockdown of Sirt3 E. F, G Fluorescence imaging revealed the increasing level of LONP1 in cytoplasm after Sirt3 deficiency F with confocal microscopy

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