Table 2 Univariate and multivariate Cox regression analysis of the characteristics in peripheral blood and 3-year overall survival in the internal cohort

AJCC stage

1.364 (1.052–1.769)

N status

1.706 (1.229–2.368)

1.610 (1.116–2.322)

CD4 to CD8 ratio

1.350 (0.544–3.351)

Subsets

 CD4⁺T_N

0.990 (0.958–1.023)

 CD4⁺T_CM

0.983 (0.935–1.034)

 CD4⁺T_TM

0.908 (0.812–1.016)

 CD4⁺T_EM

1.050 (1.005–1.097)

 CD4⁺T_EMRA

1.021 (0.954–1.093)

 CD8⁺T_N

1.010 (0.972–1.049)

 CD8⁺T_CM

1.115 (1.006–1.236)

 CD8⁺T_TM

1.026 (0.946–1.112)

 CD8⁺T_EM

1.020 (0.981–1.060)

 CD8⁺T_EMRA

0.980 (0.954–1.006)

 Treg

0.706 (0.485–1.026)

Activation

 HLA-DR⁺CD4⁺T

1.121 (0.983–1.279)

 CD38⁺HLA-DR⁺CD4⁺T

0.726 (0.302–1.744)

 HLA-DR⁺CD8⁺T

0.951 (0.862–1.048)

 CD38⁺HLA-DR⁺CD8⁺T

1.062 (0.908–1.242)

IMs

 PD-1⁺CD4⁺T

1.067 (0.868–1.312)

 PD-L1⁺CD4⁺T

0.305 (0.083–1.116)

 TIM-3⁺CD4⁺T

1.170 (0.481–2.848)

 CTLA-4⁺CD4⁺T

1.581 (0.308–8.114)

 LAG-3⁺CD4⁺T

0.180 (0.020–10,631)

 BTLA⁺CD4⁺T

0.902 (0.778–1.045)

 PD-1⁺CD8⁺T

0.911 (0.791–1.049)

 PD-L1⁺CD8⁺T

3.143(1.321–7.477)

4.309 (1.738–10.682)

 TIM-3⁺CD8⁺T

0.135 (0.039–0.463)

0.134 (0.037–0.485)

 CTLA-4⁺CD8⁺T

1.343 (0.848–2.127)

 LAG-3⁺CD8⁺T

0.264 (0.077–0.903)

 BTLA⁺CD8⁺T

0.967 (0.762–1.227)