Fig. 7

Cortical caspase-3 at mRNA level and activated caspase-3 (cleaved) at the protein level. A Brain cortical caspase-3 mRNA expression (N = 5–7 per group). B Representative merge images of DAPI—DNA blue staining of cells' nuclei and caspase-3 protein-red staining in SBN/y and SBH/y at three-time points: Baseline, 6 months, and 9 months on RD or HSD. Scale bar: 50 µm. C IF analysis of the cortical caspase-3 protein expression (N = 6–7 per group). Mean ± SEM. Three-way ANOVA was followed by Tukey’s multiple comparison test. ^***P < 0.001