Fig. 6

Fut2 interacts with MCAM and mediates the fucosylation of MCAM. A, B Co-immunoprecipitation experiments showed structural interaction between Fut2 and MCAM. C Immunoprecipitation experiments showed the expression of MCAM with or without biotinylated UEA-I in colon tissues of WT and Fut2^△IEC mice. D The typical western blot image of MCAM expression in vector and MCAM overexpression SW480 cell line with Fut2 overexpressed. E Immunoprecipitation experiments showed the expression of MCAM with or without biotinylated UEA-I in control and MCAM overexpression SW480 cell line with Fut2 overexpressed (with or without peracetylated 2-F-Fuc treated). Data are expressed as mean ± SEM. The data come from three independent experiments. (MCAM melanoma cell adhesion molecule, UEA-I Ulex Europaeus Agglutinin-I, WT wild type, 2-F-Fuc 2-fluorofucose, SEM Standard Error of Mean)