Fig. 3
Fut2 inhibits cell proliferation of SW480 and HCT116 cells. A The relative mRNA expression of Fut2 in control and fut2 overexpressed SW480 and HCT116 cells was detected by qPCR (n = 3 for per group). B The relative protein expression of Fut2 in control and fut2 overexpressed SW480 and HCT116 cells was detected by western blot (n = 3 for per group). C The typical images of SW480 and HCT116 cells stained with UEA-I. D The plate clone formation experiment of SW480 and HCT116 cells in control and Fut2-overexpression group. E CCK8 assays of SW480 and HCT116 cells in control and Fut2-overexpression group. F The typical images of transwell migration assay in control and fut2 overexpressed SW480 and HCT116 cells. G The typical images of transwell matrigel invasion assay in control and fut2 overexpressed SW480 and HCT116 cells. H, I Wound-healing assay at 48 h in control and fut2 overexpressed SW480 and HCT116 cells. Data are expressed as mean ± SEM. The data come from three independent experiments. In all panels: *p < 0.05, ***p < 0.001. (CCK8 Cell Counting Kit-8, SEM Standard Error of Mean)