Fig. 1

Fut2 is down regulated in CRC and correlates with poor prognosis. A Expression of Fut2 mRNA (including 275 CRC tissues and 45 normal colon tissues) from TCGA database was first analyzed. B Amplification of Fut2 gene copy number associated with good disease-free survival in TCGA cohort (n = 135, log-rank test, P = 0.047). C ROC curves for classifying CRC tissues versus normal colon tissues in the TCGA database. D The proteins level of Fut2 in colon tissues extracted from normal and colorectal cancer patient (n = 4 per group). E The relative mRNA expression of Fut2 in control and colorectal cancer patient colon tissues was detected by qPCR (n = 4 for per group). F The proteins level of Fut2 in colon tissues extracted from control mice and AOM/DSS mice (n = 3 per group). G The relative mRNA expression of Fut2 in control and AOM/DSS mice colon tissues was detected by qPCR (n = 5 for per group). H The typical images of mice colon tissues stained with UEA-I. I The mean gray value of UEA-I in control mice and AOM/DSS mice (n = 5 per group). Data are expressed as mean ± SEM. The data come from three independent experiments. In all panels: *p < 0.05, **p < 0.01. (CRC colorectal cancer, TCGA The Cancer Genome Atlas, AOM/DSS azoxymethane/dextran sodium sulfate, UEA-I Ulex Europaeus Agglutinin-I, SEM Standard Error of Mean)