Fig. 5

Digital cytometry based on the gene expression profile revealed that LM patients presented high proportions of CD8+ T cells. A Bar graph representing 22 immune cell scores in patients with LM and HM calculated using CIBERSORTx. *P-value = 0.037 (Mann–Whitney). B Gene expression profiles (GEP) of secretome genes found upregulated in tumor tissue of LM patients analyzed in single-cell RNA-Seq in peripheral blood mononuclear cells (PBMCs) of NSCLC patients. GEP represents the estimated expression values (log2) calculated by CIBERSORTx using the group mode function. C Principal component analysis (PCA) demonstrating HM and LM clusters generated using the CD8+ T cells enriched expression profile. D Box-plots of CD8 naive, cytotoxic, and exhausted cell scores between LM and HM patients determined using ImmuCellAI. *P-value < 0.05. E Cell–cell communications between T cell subsets from NSCLC using the upregulated genes list enriched in LM patients (single-cell data of NSCLC T cell population from GSE99254). P-values are indicated by circle size. The average expression level of interacting molecule 1 in CD8 subset red and interacting molecule 2 in CD8 subset purple is indicated by color. Analysis was performed using CellPhoneDB. LM: low-muscularity; HM: high-muscularity