Fig. 8

Downregulation of SIRT1 repressed tumor progression of ICC cells in vitro. a Western blot analysis of H3K4ac protein level in ICC cell lines after SIRT1 knockdown. b Cell growth by CCK-8 assays of ICC cells with SIRT1 knockdown. c Cell cycle progression was measured by flow cytometry of ICC cells with SIRT1 knockdown. d Quantitation of cell cycle analysis. e Cell apoptosis was measured by flow cytometry of ICC cells with SIRT1 knockdown. f Quantitation of cell apoptosis analysis. g Transwell migration assay and matrigel invasion assay of ICC cells with SIRT1 knockdown. Scale bars, 100 μm. h Quantitation of transwell assays. i Wound healing assay of ICC cells with SIRT1 knockdown. Images of wound repair were taken at 0, 24 h after wounding. j Quantitation of wound healing assays. The distance of wound closure was shown by area at 24 h. siSIRT1-1 and siSIRT1-2 indicate SIRT1 knockdown in ICC cells. *P < 0.05, **P < 0.01, ***P < 0.001