Fig. 7
SIRT1 was selected as a downstream target of VIRMA-mediated m6A modification. a The proportion analysis for specific m6A binding sites in MeRIP-seq. TSS, transcription start site; TES, transcription end site. b KEGG analysis of down-regulated genes identified for MeRIP-seq. c Volcano figure of RNA-seq analysis. d Differential genes are generated by the intersection of sequencing results. e qRT-PCR analysis of mRNA expression of the above-mentioned differential genes in ICC cell line with VIRMA knockdown. f Western blot analysis of SIRT1 protein expression in ICC cell line with VIRMA knockdown. g IGV of SIRT1. h, i RNA pull-down analysis (h) and RIP assays (i) were employed to determine the interaction of SIRT1 and VIRMA. j Correlation analysis between SIRT1 and VIRMA in TCGA ICC dataset. *P < 0.05, **P < 0.01, ***P < 0.001