Fig. 4

mtROS inhibit HIF-1a degradation. A ODD-Luc reporter Jurkat cell line was constructed. B ODD-Luc reporter Jurkat cells were treated with cholesterol, DMOG, or MG-132 for 48 h, and luciferase activity was measured. n = 5 per group. C ODD-Luc reporter Jurkat cells were treated with cholesterol alone or combined with MT for 48 h, and luciferase activity was measured. n = 5 per group. D ODD-Luc reporter Jurkat cells were stimulated with 20 mg/ml of cholesterol for 12–48 h, and luciferase activity was measured. n = 5 per group. E Naïve T cells were polarized for 2 days in the presence or absence DMOG. The percentage of Foxp3.⁺ cells was measured with flow cytometry. n = 4 per group. F Protein levels of HIF-1a were analyzed by western blotting at day 2 of polarization. n = 4 per group. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01