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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Cholesterol suppresses human iTreg differentiation and nTreg function through mitochondria-related mechanisms

Fig. 2

Cholesterol attenuates iTreg differentiation by inducing HIF-1a expression. A Protein levels of HIF-1a were analyzed by western blotting at day 2 of polarization with different doses of cholesterol added in the culture. Expression of b-actin was used as a loading control. n = 4 per group. B Relative mRNA levels of HIF-1a, VEGF and GLUT-1 were measured by RT-qPCR. n = 4 per group. C Protein levels of HIF-1a were analyzed at day 2 of polarization in the presence or absence of PX-478. n = 4 per group. D The percentage of Foxp3.+ cells at day 2 of polarization was measured with flow cytometry. n = 4 per group. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01

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