Fig. 1

Cholesterol treatment at day 3 post plating inhibits iTreg differentiation. Naïve T cells were treated with cholesterol at the day of plating (A, C) or 3 days after plating (B, D). A, B Cellular apoptosis was measured by Annexin V/PI staining. n = 4 per group. C, D Cell viability was determined by CCK8 assay. n = 5 per group. E Naïve T cells were stimulated with cholesterol and iTreg polarization cytokines at day 3 post-plating and continued to cultivate for 2 days. Frequency of Foxp3.⁺ cells was analyzed by flow cytometry. n = 4 per group. Data are expressed as the mean ± SEM. *P < 0.05, **P < 0.01