Fig. 6

Inhibition of miR-34a-5p relieved the apoptosis of cardiomyocytes induced by lncRNA Kcnq1ot1 knockdown. A Inhibition of miR-34a-5p increased the viability of mouse cardiomyocytes with lncRNA Kcnq1ot1 knockdown. B Inhibition of miR-34a-5p decreased the inhibition rate of mouse cardiomyocytes with lncRNA Kcnq1ot1 knockdown. C Representative TUNEL staining images of mouse cardiomyocytes. Magnification: 200 × ; scale bar: 100 μm. D Inhibition of miR-34a-5p increased lncRNA Kcnq1ot1 expression in si-Kcnq1ot1-transfected mouse cardiomyocytes. E Transfection with si-Kcnq1ot1 increased miR-34a-5p expression, which was reversed by miR-34a-5p inhibition. F Inhibition of miR-34a-5p increased Sirt1 mRNA expression in si-Kcnq1ot1-transfected mouse cardiomyocytes. G Inhibition of miR-34a-5p increased Sirt1 protein expression in si-Kcnq1ot1-transfected mouse cardiomyocytes. H Inhibition of miR-34a-5p decreased Bax protein expression in si-Kcnq1ot1-transfected mouse cardiomyocytes. I Inhibition of miR-34a-5p increased Bcl-2 protein expression in si-Kcnq1ot1-transfected mouse cardiomyocytes. For A, B, and D–I, one-way ANOVA F value = 8.525, 8.525, 11.17, 16.29, 29.86, 7.684, 22.66 and 16.11, respectively. * P < 0.05, ** P < 0.01, ***P < 0.001 vs. si-NC + AMO-NC group. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. si-Kcnq1ot1 + AMO-NC group; for A, B, n = 10; for C–I, n = 3–4