Fig. 3

The effect of miR-34a-5p on Sirt1 expression in mouse cardiomyocytes. A The expression of miR-34a-5p in cardiomyocytes transfected with miR-34a-5p mimic. B Upregulation of miR-34a-5p inhibited Sirt1 mRNA expression in mouse cardiomyocytes. C Upregulation of miR-34a-5p inhibited Sirt1 protein expression in mouse cardiomyocytes. D The expression of miR-34a-5p in cardiomyocytes transfected with AMO-34a-5p. E Downregulation of miR-34a-5p increased Sirt1 mRNA expression in mouse cardiomyocytes. F Downregulation of miR-34a-5p increased Sirt1 protein expression in mouse cardiomyocytes. For A–C, *P < 0.05, **P < 0.01 vs. miR-NC group. For D–F, *P < 0.05 vs. AMO-NC group; n = 3–6