Fig. 5

Tan IIA ameliorates SIRT1-mediated ER stress in the BMECs of APP/PS1 mice. a-c Western blot (a, b) and immunofluorescence (c) analysis of the expression of SIRT1. d-l Western blot analysis of ER stress in APP/PS1 mice: e BIP; f P-PERK/ PERK; g P-eIF-2α/ eIF-2α; h P-IRE-1α/ IRE-1α; i XBP1; j PDI; k ATF6; and l CHOP. m, n Indirect immunofluorescence of P-PERK (m) and P-IRE-1α (n) in vascular endothelium of APP/PS1 mice. Scale bar, 50 μm; inset, 20 μm. Tan-L: Tan IIA (10 mg/kg/d); Tan-H: Tan IIA (20 mg/kg/d). The mean ± SD was calculated based on three separate studies. *P < 0.05, **P < 0.01, NS: no significance