Fig. 5

mRNA and protein expression of EREG. A mRNA expression of normal and tumor tissues. B mRNA expression of NHA and GBM cell lines. C Protein expression of normal and tumor tissues. D Western blot was used to detect knockdown inefficiency of EREG in the U87 and U251 cell lines. E The protein expression of EREG in normal and tumor tissues was detected by immunohistochemistry. F qRT-PCR was used to detect knockdown inefficiency of EREG in the U87 and U251 cell lines. G Protein expression of NHA and GBM cell lines.*P < 0.05, **P < 0.01, ***P < 0.001. Error bars indicate the mean ± SD