Fig. 6

Knockout of HLA-DRB5 in Caco2 cells inhibits LPS-induced IL-1α expression. A, B An HLA-DRB5 expression plasmid was introduced into Caco2 cells, and the expression of this gene and IL-1α was assessed over time using qPCR and Western immunoblotting. C, D Caco2 cells were analyzed in control, LPS treatment (10 μg/ml), HLA-DRB5, and HLA-DRB5 + LPS groups. Cells were collected at 3 h post-LPS treatment and 24 h post-HLA-DRB5 transfection to assess HLA-DRB5 and IL-1α expression by qPCR. E, F Control, HLA-DRB5 overexpression, and HLA-DRB5 knockout groups were analyzed, with cells collected 8 h post-LPS stimulation and 24 h post-HLA-DRB5 transfection to measure IL-1α expression by qPCR