Fig. 7

Flow cytometry assays to analyze the tumor immune microenvironment in PD1 combined with JK184 treated MC38 tumors. Representative images of A tumor-infiltrating lymphocytes, B CD3⁺ T cells, C CD8⁺ T cells, D granzyme B⁺ T cells, E IFN-γ⁺ T cells, F CD4⁺ FOXP3⁺ T cells, and G myeloid-derived suppressor cells (MDSCs). The relative quantitative analysis was performed by the ratio of tumor-infiltrating immune cells to total alive cells in the prepared cell suspension. T tests were used to determine statistical significance of the differences as indicated: *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001, ns not significant