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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: A drug screening to identify novel combinatorial strategies for boosting cancer immunotherapy efficacy

Fig. 4

Transcriptional signatures of T cells after JK184 treatment. A Heatmap showing differentially expressed genes between JK184-treated T cells and nontreated T cells (WT). Each sample group comprised triplicates. B Bubble plot of the top 25 enriched KEGG pathways in JK184-treated T cells versus nontreated T cells. The size and color of the circles in the plots are scaled according to the enrichment score calculated by the number of differentially expressed genes and P value. C The volcano plot demonstrating fold changes in gene expression in JK184-treated T cells compared with nontreated T cells. Red dots and green dots represent significantly upregulated genes and downregulated genes respectively, and gray dots represent genes that are statistically nonsignificant. Values are presented as the log2 of the tag counts. D Real-time PCR to confirm the expression of genes marked in the volcano plot. Each sample group comprised triplicates. E Representative GSEA enrichment plot demonstrating significant upregulation of KEGG MAPK signaling pathway-related genes (FDR = 0.066 and NES = 1.615), KEGG calcium signaling pathway (FDR = 0.11 and NES = 1.471) and REACTOME Toll-like receptor cascade-related genes (FDR = 0.084 and NES = 1.733) in JK184-treated T cells versus nontreated T cells. T tests were used to determine statistical significance of the differences in (D). *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001

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