Fig. 2

Identification of BML284/PPP/JK184 as a potent adjutant of CAR T cells in vitro. A MDA-MB-231 and HCT116 cells were analyzed by flow cytometry for Annexin V/7AAD staining after treatment with BML284/PPP/JK184 (1 µM) for 24 h. B CD19 or B7-H3 CAR T cells cocultured with tumor cells at an E:T ratio of 1:1 combined with BML284/PPP/JK184 or DMSO. The secretion of perforin and granzyme B by CAR T cells was detected and compared. C The cytotoxicity of multiple treatment groups on MDA-MB-231 and HCT116 cells in this coculture assay was monitored using the RTCA system. D and E HCT116 spheroids were incubated with DMSO, BML284/PPP/JK184, B7-H3 CAR T cells, and BML284/PPP/JK184 plus B7-H3 CAR T cells respectively, and pictures were captured by a confocal microscope (Zeiss 880). Scale bars, 50 µm. In Fig. 2C, *P < 0.05, ***P < 0.001, by a two-tailed Student’s t test. Each experiment was performed in triplicate