Fig. 5

A Western blot analysis of FASN expression in LNCaP and C4-2 cells before and after treatment with TVB-2640 50 μM for 24 h and 48 h. Data were normalized based on control relative abundance. Statistical significance has been calculated with two saples t-test (treated and control) and reported as p** < 0.01. B Histograms reporting absorbance values of red oil assay in LNCaP cells before and after treatment with TVB-2640 50 μM for 72 h. Statistical significance calculated with two saples t-test and reported as p** < 0.01. C–D Effects of TVB-2640-related on propidium iodide (PI) incorporation, LNCaP and C4-2 cells were treated with TVB-2640 at 50 μM for 24 h. Graphs of PI distribution show the alive cells (green) and death cells (red). E–F Effects of TVB-2640-related on cell cycle regulation. LNCaP and C4-2 were treated with TVB-2640 at 50 μM for 24 h. Graphs show subG1, G0/G1, S and G2M phases. G Histograms reporting absorbance values of colony formation assay on LNCaP cells before and after treatment with TVB-2640 50 μM for 72 h. Statistical significance has been calculated with two saples t-test and reported as p** < 0.01