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Fig. 9 | Journal of Translational Medicine

Fig. 9

From: A machine learning framework develops a DNA replication stress model for predicting clinical outcomes and therapeutic vulnerability in primary prostate cancer

Fig. 9

Knockdown of FEN1 and RFC5 inhibits cell growth and promotes apoptosis. Levels of FEN1 and RFC expression in C4-2B and PC-3 are decreased by siRNA knockdown as measured in the A real-time qPCR and B Western blot analysis. Comparison of cell growth among the control, FEN1, and RFC5 knockdown groups in C4-2B and PC-3 via C CCK-8 and D colony formation assays. E Measurement of cell apoptosis in control, FEN1, and RFC5 knockdown groups by flow cytometry. Cells are stained with Annexin V-fluorescein 5-isothiocyanate/PI assay. The asterisks represented the statistical P-value (*P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001)

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Journal of Translational Medicine

ISSN: 1479-5876

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