Fig. 4

AFAP1L1 facilitates EMT of GC cells. A Pearson correlation analysis of AFAP1L1 and EMT markers (epithelial marker: E-cadherin, EPCAM; mesenchymal markers: vimentin, N-cadherin) in GC using data from TCGA STAD database. B qRT-PCR analysis of AFAP1L1 and EMT markers in AGS^shAFAP1L1, MKN74^AFAP1L1 and their control cells. C Western blot analysis of AFAP1L1 and EMT markers in AGS^shAFAP1L1, MKN74^AFAP1L1 and their control cells. D Double immunofluorescence staining of E-cadherin (red) and vimentin (green) in AGS^shAFAP1L1, MKN74^AFAP1L1 and their control cells. Nuclei was stained with DAPI (blue). Scale bars, 10 μm. E The representative IHC images from serial sections of GC samples showed AFAP1L1, E-cadherin and vimentin expression in ANGT and GC tissues with high or low AFAP1L1 expression. ***P < 0.001