Fig. 7

D-mannose restrained osteoclastogenesis through DC-STAMP mediated osteoclast fusion. a Volcanic map of RNA sequencing. The green dots on the left indicated there were 475 downregulated RNAs and 544 upregulated RNAs in OCM + Man group compared with OCM group. b GO gene enrichment map. c Gene expression heat map. d Schematic illustration of osteoclast fusion mechanism. e Relative expression of osteogenesis-relating genes including mNfatc1, mC-fos, and mDc-stamp of RAW264.7 cells. f Relative expression of osteogenesis-relating genes including rNfatc1, rC-fos, and rDc-stamp of rBMMs. g Western blot analysis of NFATc1, c-Fos, and DC-STAMP expression of RAW264.7 cells. h Western blot analysis of NFATc1, c-Fos, and DC-STAMP expression of rBMMs