Fig. 5From: β-glucan protects against necrotizing enterocolitis in mice by inhibiting intestinal inflammation, improving the gut barrier, and modulating gut microbiotaEffects of β-glucan to the tight function proteins in mice with NEC. a, b The expression of ZO-1 and Occludin was determined in the intestinal tissues of the three groups by immunohistochemical staining. Magnification: × 200. c–f Protein expression of the ZO-1, Occludin, and Claudin-1 was identified in the intestinal tissues of the three groups using western blot. n = 4. Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001Back to article page