Fig. 4

The binding mode between the β-glucan and the TLR4 using molecular docking. a–c Molecular docking simulation of β-glucan into the active site of TLR4. d The results of the binding mode analysis. The meanings of colors in specific amino acids binding with TLR4 are as follows: red stands for negatively charged acidic amino acids, dark blue stands for positively charged basic amino acids, light blue stands for polar amino acids, and green stands for hydrophobic amino acids. e The results of LigPlus analysis of hydrogen bonds and hydrophobic interactions. The green dashed line indicates hydrogen bonds, and the red eyelashes indicate hydrophobic interactions, which are directional