Fig. 3

Effects of β-glucan to the TLR4-NF-B pathway in mice with NEC. a The relative mRNA expression levels of TLR4. n = 6–8 in each group. b–d Protein expression of TLR4 and NF-B in the three groups determined using western blot. n = 4. e, f TLR4 and NF-B were assessed in the intestinal tissues of the three groups by immunohistochemical staining. Magnification × 200. Data are expressed as the mean ± SD, *P < 0.05, ***P < 0.001