Fig. 9
The mRNA folding analysis and PspA1-5c+p construct in-silico cloning into the pET28a( +) expression vector. a The mRNA secondary structure of the PspA1-5c+p construct with Gibbs free energy of -445.5 kcal.mol−1. b In-silico cloning of the final PspA1-5c+p construct sequence into the pET28a ( +) expression vector. The yellow part represents the gene encoding PspA1-5c+p, and the black circle represents the vector backbone