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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: In-silico design and evaluation of an epitope-based serotype-independent promising vaccine candidate for highly cross-reactive regions of pneumococcal surface protein A

Fig. 2

Schematic representation of the final PspA1-5c+p vaccine candidate. To cover the maximum cross-reactivity between PspA two families and the diversity of all PRD groups, the 614 amino acid long peptide sequence contains immunodominant B-cell and T-cell epitopes as the truncated domain of the CDRs and the highly conserved region of NPB and repetitive motifs of PRD group. The CDR regions of the PspA family1 (orange) at the amino-terminal end are connected to the multi-epitope sequence of the PRD (green) via an EAAAK linker (yellow). PspA family 2 CDR regions (blue) are also linked using an EAAAK linker (yellow) together with PRD in the carboxy-terminal of the construct. Two 6 × His tags are added to the amino and carboxyl terminus of the construct for purification and identification purposes. For the cloning of the PspA1-5c+p construct into the pET28a vector, the restriction enzyme sites (NcoI and XhoI) are considered

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