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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: M2 tumor-associated macrophage mediates the maintenance of stemness to promote cisplatin resistance by secreting TGF-β1 in esophageal squamous cell carcinoma

Fig. 5

TGF-β1 secreted by M2-TAMs contributes to cisplatinum resistance by promoting stemness. A CCK-8 assay was used to detect the cisplatin IC50 values of ESCC sphere cells and parental cells. B CCK-8 assay analyzed the changes of the absorbance at 450 nm of EC109 sphere cells and EC9706 sphere cells compared with parental cells after 4ug/ml cisplatin for different time points (24, 48, 72 h). C Plate cloning-formation assay detected the proliferation abilities of ESCC cells in 4ug/ml cisplatin medium. D FCM assay was used to analyze the changes of apoptosis level of ESCC parental cells and sphere cells in 4ug/ml cisplatin. E FCM assay was used to analyze the changes of apoptosis rates of ESCC sphere cells and parental cells treated with rhTGF-β1 in 4ug/ml cisplatin medium. F CCK-8 assay detected the changes of absorbance at 450 nm of ESCC sphere cells in 4ug/ml cisplatin medium after treated with M2-CM, M2-CM + SB431542 for different time points (24, 48, 72 h). G Plate cloning-formation assay detected the changes of the cell proliferation ability of ESCC sphere cells in 4ug/ml cisplatin medium after pretreated with M2-CM, M2-CM + SB431542 for 24 h. H FCM assay detected the changes of apoptosis rate of ESCC sphere cells in 4ug/ml cisplatin medium after pretreated with M2-CM, M2-CM + SB431542 for 24 h. All experiments were repeated three times, and the results were expressed as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001

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