Fig. 2

TGF-β1 derived from M2-TAMs caused ESCC cells to resist cisplatin. A, B qRT-PCR and western blot detected the mRNA and protein levels of TGF-β1 in M2-TAMs and M0-TAMs C The expression levels of TGF-β1 in the supernatant of co-cultured and non-co-cultured systems was determined by Elisa. D, E qRT-PCR, western blotting analyzed the TGF-β1 mRNA and protein levels of ESCC cells and M2-TAMs in co-cultured and non-co-cultured systems. F CCK-8 detected the cisplatin IC50 values of EC109 and EC9706 cells after 10 ng/ml rhTGF-β1 pretreatment. G Plate clone detected the proliferation abilities of EC109 and EC9706 cells in 4ug/ml cisplatin medium when pretreated with 10 ng/ml rhTGF-β1 for 24 h. H FCM revealed the apoptosis of EC109 and EC9706 cells in 4ug/ml cisplatin medium after pretreated with 10 ng/ml rhTGF-β1 for 24 h. All results were repeated three times and expressed as mean ± SD, and there was no significant difference in ns, *P < 0.05, **P < 0.01, ***P < 0.001