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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: M2 tumor-associated macrophage mediates the maintenance of stemness to promote cisplatin resistance by secreting TGF-β1 in esophageal squamous cell carcinoma

Fig. 1

M2-TAM mediates the resistance of esophageal squamous cell carcinoma to cisplatin A Schematic diagram of the induction of M2-TAMs. THP-1 cells were induced by PMA for 24 h to become M0-TAMs, and then induced by IL-4 and IL-13 for 36 h to become M2-TAMs. B Changes in cell morphology during induction of THP-1 to M2-TAM. Scale bar:100 µm. C qRT-PCR detectd the relative M2 macrophage markers in M0-TAMs and M2-TAMs. D, F CCK-8 detected the changes of IC50 values of EC109 cells and EC9706 cells pretreated with M2-CM for 24 h. E, G Plate colony formation experiments showed the proliferation abilities of EC109 cells and EC9706 cells in 4ug/ml cisplatin mediumafter pretreated with M2-CM. H FCM analyzed the apoptosis rates of EC109 and EC9706 cells in 4ug/ml cisplatin medium after pretreated with M2-CM for 24 h. I Tumor images of 3 groups of mice: PBS liposome + CDDP group, chlorophosphate liposome + CDDP group, and M2-TAM + CDDP group. J Quality comparison of three groups of tumors. K Volume growth curves of three groups of tumors. All results were repeated three times and expressed as mean ± SD, there was no significant difference in ns, *P < 0.05, **P < 0.01, ***P < 0.001

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