Fig. 3
From: Aa-Z2 triggers ROS-induced apoptosis of osteosarcoma by targeting PDK-1
Aa-Z2-induced ROS promoted the apoptosis cascade and blocked the PI3K/Akt/mTOR signalling pathway in OS cells. A, B Apoptosis cascade detection with WB in Aa-Z2-treated 143B and HOS cells. C, D PI3K/Akt/mTOR signalling pathway assessment with WB in Aa-Z2-treated 143B and HOS cells. E, F Autophagy related pathway detection with WB in Aa-Z2-treated 143B and HOS cells. Mean ± SD (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)