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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Aa-Z2 triggers ROS-induced apoptosis of osteosarcoma by targeting PDK-1

Fig. 2

Aa-Z2 suppressed colony formation ability and induced cell apoptosis, along with increased levels of ROS in OS cells. A, B Colony formation assay for Aa-Z2-treated 143B and HOS cells. C, D Transmission electron microscopy for apoptotic bodies of 0.8 μM Aa-Z2-treated 143B and HOS cells. E, F DCFH-DA assay for ROS levels in Aa-Z2-treated 143B and HOS cells. G, H Flow cytometry detection of apoptosis when 143B and HOS cells were treated with Aa-Z2 & NAC. I, J Flow cytometry detection of G2/M phase cells when 143B and HOS cells were treated with Aa-Z2 & NAC. Mean ± SD (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)

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