Fig. 1

Aa-Z2 inhibited cell viability and induced G2/M phase arrest in OS cells. A The synthesis and structure of Aa-Z2. B The antiproliferative effect of Aa-Z2 on different cell lines was determined by CCK-8 assay. C, D Comparison of the IC50 values of Aa-Z2 in four OS cell lines and four nontumor cell lines. E The antiproliferative effect of ATO on 143B and HOS cells. F 143B and HOS cells were treated with Aa-Z2 and ATO for 24 h at a concentration of 0.8 μM, and cell viability was measured by CCK-8. G, H The cell cycle of 143B and HOS cells was analysed after 0.8 μM Aa-Z2 or ATO treatment for 24 h. I, J ROS levels of 143B and HOS cells were analysed after 0.8 μM Aa-Z2 or ATO treatment for 24 h