Fig. 9

LRG1 promoted malignant phenotypes in PCa cells and HUVEC tube formation. A, B The overexpression efficiency of LRG1 after transfection with targeted LRG1 (PCDH-LRG1) or negative control (CTR) was confirmed by RT-qPCR and western blot in DU145, LNCaP and PC-3 cells. C, D Overexpression of LRG1 enhanced proliferation and colony formation of DU145, LNCaP and PC-3 cells, especially PC-3 and LNCaP cell lines. *p < 0.05; **p < 0.01; ***p < 0.001 (C two-way ANOVA, D Student, s t test). E Wound healing experiments on PC-3 and LNCaP cells showed that LRG1 overexpression significantly increased the percentage of wound healing at 0 h and 48 h in experimental groups compared with the control group. F The transwell experiment of PC-3 and LNCaP showed that the invasion of migratory cells was significantly enhanced in the LRG1 overexpressing group compared with the control group (Vector). G LNCaP overexpressed exosomes derived from cell lines may be involved in prostate cancer angiogenesis. Representative images showed HUVEC tube formation at 4 h and 8 h after treated with the same amount of exosome. *p < 0.05; **p < 0.01; ***p < 0.001 (Student, s t test)