Fig. 4

Differentiation and infiltration of T cells were influenced by CCCs. A, B Cell annotation of CD4 + T cells and CD8 + T cells by t-SNE plot, respectively. C Monocle analysis of CD4 + T cells and CD8 + T cells, which are ordered by pseudotime. D Network plot displaying the interaction strength and numbers of communication between CD4 + T cells, CD8 + T cells and tumor cells respectively. Welch’s t test: *P < 0.05; **P < 0.01; ***P < 0.001. E, F Expression conditions of CCCs molecules in the differentiation of CD4 + T cells and CD8 + T cells, respectively. G Heatmap demonstrating that CCCs molecules correlate with T cell infiltration in tumors