Fig. 8

Silencing circ_0001789 suppresses the tumorigenesis and metastasis of GC cells in vivo. A Tumor volume of GC cells transfected with sh-NC and sh-circ_0001789 in nude mice. B Tumor weight of xenografts from nude mice was measured at the end of the experiment in the sh-NC and sh-circ_0001789 groups. C Reverse transcription-quantitative PCR analysis of the expression level of circ_0001789, microRNA-140-3p and PAK2 in the xenografts of nude mice. D Immunohistochemistry was performed to detect the expression of Ki-67, PAK2, N-cadherin and CD34 in the xenografts of nude mice in the sh-NC and sh-circ_0001789 groups. E Hematoxylin and eosin staining of lung metastatic nodules in nude mice of the sh-NC and sh-circ_0001789 groups. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. circ, circular RNA; GC, gastric cancer; NC, negative control; sh, small hairpin; PAK2, p21 activated kinase 2