Skip to main content
Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Circular RNA 0001789 sponges miR-140-3p and regulates PAK2 to promote the progression of gastric cancer

Fig. 6

circ_0001789 sponges miR-140-3p to upregulate PAK2 expression. A The ‘Targetscan’, ‘miRDB’, ‘TarBase’ and ‘UP-GEPIA’ databases were employed to predict the downstream target mRNAs of miR-140-3p: ADAM10, PAK2, NFYA, CAND1, MED13 and USP31. B RT-qPCR was used to detect the candidate mRNA levels of ADAM10, PAK2, NFYA, CAND1, MED13 and USP31 in AGS and HGC27 cells transfected with miR-NC or miR-140-3p mimics. C The Cancer Genome Atlas database analysis of GC samples and normal gastric samples showed upregulation of PAK2 in GC. D RT-qPCR revealed the expression levels of PAK2 in normal gastric mucosa samples of healthy controls (n = 50) and GC tissues (n = 70). E Kaplan–Meier plot showing the overall survival of patients with GC exhibiting high or low expression of PAK2 (n = 35 in each group). F and G Pearson’s correlation analysis revealed a positive correlation between PAK2 and circ_0001789, and a negative correlation between PAK2 and miR-140-3p expression (r > 0.6, P < 0.01). H Western blot analysis of the PAK2 level in GC cell lines (AGS, HGC27, MKN-45 and MKN-74) and normal gastric epithelial cells (GES-1). I Schematic diagram of the binding site between miR-140-3p and the 3’ untranslated region of PAK2 mRNA, and the mutated binding sequence. J Dual luciferase reporter assay in AGS and HGC27 cells using a reporter containing a wild-type or a mutated binding site in the presence of miR-NC or miR-140-3p mimic. K PAK2 expression levels in different groups of AGS and HGC27 cells (sh-NC, sh- circ_0001789 or circ_0001789 + miR-140-3p inhibitor) were detected by western blotting. *P < 0.05, **P < 0.01, ***P < 0.001. circ, circular RNA; miR, microRNA; PAK2, p21 activated kinase 2; ADAM10, a disintegrin and metalloproteinase domain-containing protein 10; NFYA, nuclear transcription factor Y subunit α; CAND1, cullin associated and neddylation dissociated 1; MED13, mediator complex subunit 13; USP31, ubiquitin specific peptidase 31; RT-qPCR, reverse transcription-quantitative PCR; NC, negative control; GC, gastric cancer

Back to article page