Fig. 3

Knockdown of circ_0001789 impairs the malignant features of GC cells. (A) Two GC cell lines (AGS and HGC27) with high expression of circ_0001789 were selected for gene silencing using shRNAs targeting circ_0001789. B The RAB11 family interacting protein 1 mRNA levels in AGS and HGC27 cells transfected with sh-NC or sh-circ_0001789 were detected by reverse transcription-quantitative PCR. C Cell Counting Kit-8 proliferation assay in AGS and HGC27 cells transfected with sh-NC or sh-circ_0001789. D and E Cell migration and invasion assays in AGS and HGC27 cells transfected with sh-NC or sh-circ_0001789. F Tube formation assay in human umbilical vein endothelial cells incubated with exosome samples from AGS and HGC27 cells transfected with sh-NC or sh-circ_0001789. G Protein levels of E-cadherin, N-cadherin, vimentin and VEGF-A in AGS and HGC27 cells transfected with sh-NC or sh-circ_0001789. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. circ, circular RNA; GC, gastric cancer; sh, small hairpin; NC, negative control