Fig. 2

circ_0001789 participates in cell-to-cell communication via exosomes in GC cells. A A representative TEM image of exosomes in the serum samples of normal individuals and patients with GC. B The diameter of exosomes was analyzed by TEM. C Relative circ_0001789 expression level in exosome samples of healthy controls (n = 50) and patients with GC (n = 70). D Receiver operating characteristic curve analysis of the specificity and sensitivity of exosomal circ_0001789 as a diagnostic marker for GC. E Western blot detection of exosomal markers (CD63 and tumor susceptibility gene 101) in the exosome samples of GC cell lines (AGS, HGC27 and MKN-45) and normal gastric epithelial cells (GES-1). F The relative circ_0001789 levels in exosomal samples of GC cell lines (AGS, HGC27 and MKN-45) and normal gastric epithelial cells (GES-1) were detected by RT-qPCR. G Relative circ_0001789 expression level in cells and exosomal samples of MKN-45 cells transfected with empty vector or circ_0001789 expression vector. H AGS and HGC27 cells were incubated with exosomes from MKN-45 cells transfected with empty vector (vector-exo) or circ_0001789 expression vector (circ_0001789-exo) for 48 h, and the relative circ_0001789 levels were determined by RT-qPCR. I Cell Counting Kit-8 proliferation assay in AGS and HGC27 cells treated with vector-exo or circ_0001789-exo. J and K Cell migration and invasion assays in AGS and HGC27 cells treated with vector-exo or circ_0001789-exo. L Tube formation assay in human umbilical vein endothelial cells treated with vector-exo or circ_0001789-exo. M Protein levels of E-cadherin, N-cadherin, vimentin and VEGF-A in AGS and HGC27 cells treated with vector-exo or circ_0001789-exo. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. circ, circular RNA; GC, gastric cancer; TEM, transmission electron microscopy; RT-qPCR, reverse transcription-quantitative PCR