Fig. 2

cNDC80 induces GBM cell proliferation, migration, and invasion in vitro. A The expression levels of cNDC80 in GBM cells and NHAs were investigated using qRT–PCR. B Designated shRNAs for cNDC80 are shown schematically at splice junctions. C In U87 and pGBM-1 cells transduced with either cNDC80-targeting shRNA-3 or a non-targeted control shRNA, the expression of cNDC80 was measured by qRT–PCR. D–F CCK-8, colony formation, and EdU were used to identify the cell proliferation capacities of transfected U87 and pGBM-1 cells. G Transwell for cell migration and invasion studies after expression vector or shRNA transfection of glioma cells. H Using the wound healing assay, the migration of GBM cells was evaluated. I Following transfection in GBM cells, N-cadherin, Vimentin, and CyclinD1 were analyzed using Western blots. Each experiment was conducted three times, and the findings are shown as mean ± SD. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)