Fig. 5From: Gene’s expression underpinning the divergent predictive value of [18F]F-fluorodeoxyglucose and prostate-specific membrane antigen positron emission tomography in primary prostate cancer: a bioinformatic and experimental studyImmunofluorescence staining and quantification analysis of PSMA expression patterns in LANCaP cells with different grades of aggressiveness. Representative confocal microscopy images of LNCaP-30 (solid line) and LNCaP-80 (dashed line) stained with anti-PSMA (green fluorescence) and DAPI (nuclei, blue fluorescence) in permeabilized (A) or intact (C) cells. Quantification of PSMA fluorescence in permeabilized (B) or intact (D) cells. Fluorescence was quantified by using ImageJ analysis software. Values of integral fluorescence cell are evaluated in fields containing at least 5 cells each, and are expressed as means ± SD. n = 3 experiments per group. ns not significantBack to article page