Fig. 8
SAMHD1 silencing synergized with radiotherapy to increase macrophage M1 polarization and CD8+ T cell infiltration. A The flow cytometry gate strategy of macrophages. B Representative flow cytometry of MHC-IIhighCD86+ macrophages in spleens and tumors. C, D Quantitative analysis of MHC-IIhighCD86+ macrophages in spleens and tumors. E, F Representative flow cytometry of CD8+ T cells in tumors. Quantitative analysis of CD8+ T cells in tumors. G Representative IHC staining (CD3 and CD8) of tumor tissues. Scale bar: 400 μm. N ≥ 3; *, P < 0.05; **, P < 0.01; ***, P < 0.001