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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: SAMHD1 silencing cooperates with radiotherapy to enhance anti-tumor immunity through IFI16-STING pathway in lung adenocarcinoma

Fig. 5

SAMHD1 silencing collaborated with radiotherapy to induce ssDNA accumulation and activate TBK1-IRF3-IFN-I signaling. A Confocal images were performed to detect the accumulation of ssDNA in cytoplasm in H1299 cells after radiation and transfecting siSAMHD1. B The accumulation of ssDNA in cytoplasm after radiation and siSAMHD1 transfection was detected by flow cytometry. The cytosolic ssDNA was evaluated by MFI. C TBK1-IRF3 pathway protein levels in H1299 and A549 cells after transfection and radiation were detected by immunoblotting. D The mRNA levels of SAMHD1, IFNβ, CCL5 and CXCL10 after SAMHD1 silencing and radiation in H1299 cells were detected by qPCR. E The mRNA levels of SAMHD1, IFNβ, CCL5 and CXCL10 after SAMHD1 overexpression and radiation in A549 cells were detected by qPCR. N = 3; *, P < 0.05; **, P < 0.01; ***, P < 0.001

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