Fig. 3

SAMHD1 silencing inhibited tumor growth and promoted macrophage M1 polarization. A C57BL6 mice were injected with LV-NC and LV-shSAMHD1 infected LLC cells. On the 20th day of injection, the tumors were collected for photograph. B The tumor volumes were measured every 2 days and depicted in the line chart, N ≥ 8. C Mice survival was recorded for KM curves, N ≥ 9. D Quantitative analysis of MHC-II⁺CD86⁺ macrophages in tumors, N ≥ 4. E The M1 related molecules Mhc-II and Cd86 mRNA expression in co-cultured RAW264.7 cells were detected by qPCR. F The surface expression of MHC-II was detected by flow cytometry and analyzed by MFI. G Representative flow cytometry of CD86⁺ cells. Quantitative analysis of CD86⁺ macrophages. N = 3; *, P < 0.05; **, P < 0.01; ***, P < 0.001