Fig. 2

SAMHD1 silencing activated IFI16 and TBK1-IRF3-IFN-I pathway in LUAD cells. A, B The mRNA levels of SAMHD1 and IFI16 were detected by qPCR in H1299 and H1975 cells. C The translocation of IFI16 from nucleus to cytosol in H1299 cells was detected with confocal. D IFI16 and TBK1-IRF3 pathway protein levels in H1299 and H1975 cells were detected by immunoblotting. E, F The mRNA levels of IFNβ, CCL5 and CXCL10 in H1299 and H1975 cells were detected by qPCR. G The mRNA levels of Ifnβ, Ccl5 and Cxcl10 in LLC cells were detected by qPCR. Scale bar: 40 μm. N = 3, *, P < 0.05; **, P < 0.01; ***, P < 0.001